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. 2016 Mar 8;9:18. doi: 10.3389/fnmol.2016.00018

FIGURE 6.

FIGURE 6

The protective R1398H LRRK2 variant increases axonal length in primary cortical neuronal cultures. Rat primary neurones were transfected with wild-type LRRK2 or the indicated variants prior to fixation and staining for axonal tau protein after 7 DIV. (A) Shows representative images of LRRK2-transfected neurones. (B) Quantification of the number of axon branches per neurone reveals no effect of R1441G or R1398H mutations (WT, n = 49; R1398H, n = 26; R1441G, n = 38; R1398H/R1441G, n = 31). (C) By contrast, R1398H increases axonal length and rescues the decrease in length caused by the pathogenic R1441G mutant (WT, n = 49; R1398H, n = 32; R1441G, n = 56; R1398H/R1441G, n = 39). (D) All LRRK2 mutant constructs were expressed at an equivalent level to wild-type LRRK2. Scale bar: 30 μm. p-values from post hoc Bonferroni tests are shown. Error bars represent the standard error of the mean.