Table 1.

Astral microtubule dynamics in CLB4 and Δclb4 cells

	CLB4 (n = 14)	Δclb4 (n = 14)
Rates (μm/min)
Growing	1.25 ± 0.35	1.56 ± 0.38	p < 0.0001
Shrinking	1.91 ± 0.68	2.28 ± 0.93	p < 0.0001
Frequencies (event/sec)
Catastrophe	0.0183 ± 0.0036	0.0198 ± 0.0046	p = 0.36
Rescue	0.0224 ± 0.0057	0.0233 ± 0.0036	p = 0.59
Event duration (min)
Growing	0.88 ± 0.34	0.95 ± 0.35	p = 0.17
Shrinking	0.64 ± 0.19	0.69 ± 0.25	p = 0.21
Pausing	0.58 ± 0.12	0.56 ± 0.13	p = 0.83
Event length changes (μm)
Growing	1.10	1.48
Shrinking	1.23	1.57
Total times (%)
Growing	53.3	54.2
Shrinking	38.1	43.9
Pausing	8.6	1.9
Average length (μm)	0.98 ± 0.13	1.84 ± 0.38	p < 0.0001
aMT-cortex contact (% of time)	60.9	87.6

For CLB4 and Δclb4 cells, 14 time-lapse sequences of 12,045 or 10,125 sec were obtained with the spinning-disk confocal microscope. Frequencies of catastrophes and rescues were calculated in each time-lapse sequence, and the averages of them were shown (Kosco et al. 2001). Event length changes equal mean event rates times mean event durations. P values were by unpaired t test.