Figure 1.

The pattern of recruitment of the members of the SRC family, SRC-1, GRIP1, and AIB1, on the promoters of the HRE-containing genes EBAG9 in T-47D cells (A) and PSA in LnCAP cells (B). Cells were grown in phenol red-free medium (DMEM for T-47D and RPMI-medium 1640 for LnCAP cells) supplemented with 10% charcoal-dextran-stripped FBS for at least 3 d, and left untreated or treated with 100 nM of E2 or 100 nM of DHT for 45 min. ChIP assays and ChIP/Re-IP experiments were performed using specific antibodies against SRC-1, GRIP1, and AIB1.