Subject area |
Proteomics |
More specific subject area |
Plant proteomics |
Type of data |
LC-ESI MS/MS data tables and electron microscopy images |
How data was acquired |
Raw data by nanoUHLPC-MS/MS and ESEM. |
Processed MS/MS data by sequence database searching. |
Data format |
Mascot.mgf files, also deposited as MS/MS raw files |
Experimental factors |
Trichomes were isolated from frozen leaves of Artemisia annua cultivar Artemis. Proteins were extracted and digested from trichome-enriched, trichome-depleted and whole leaf samples. |
Experimental features |
NanoUHPLC-MS/MS-based proteomics was applied to the analysis of trichome-enriched, trichome-depleted and whole leaf samples of Artemisia annua. Protein identification searches were performed against genomic and proteomic sequence databases for comparison of results using Mascot Daemon software. Obtained emPAI values were used for quantitative protein abundance comparisons. |
Data source location |
EMBL-EBI, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1 SD, UK and Department of Chemistry, University of Reading, Reading, Berkshire, RG6 6AD, UK |
Data accessibility |
Via the PRIDE (Proteomics Identifications Database) repository at the European Bioinformatics Institute (http://www.ebi.ac.uk/pride/), PXD000703. |