Subject area	Biology, Biochemistry
More specific subject area	Protein interactions
Type of data	Western blots
How data was acquired	SDS-PAGE, Western transfer, ECL
Data format	Raw and analysed
Experimental factors	Arabidopsis thaliana (L.) ecotype Columbia (Col-0, WT) plants and At4g01050 knock-out mutant line, trol, were grown either in dark or under 80 µmol photons m−2s−1(GL) or 250 µmol photons m−2s−1(HL), respectively. Intact Arabidopsis chloroplasts were isolated from 3–4 week old plants and analysed by using SDS-PAGE for TROL-FNR complex stability.
Experimental features	For TROL-FNR complex dynamics investigation, thylakoids were isolated from intact Arabidopsis chloroplasts, separated by ultracentrifugation into insoluble and soluble fractions, treated by nonionic detergents at different pH, analysed by SDS-PAGE, Western transfer, immunodecorated with α-FNR antibody, and finally detected by semiquantitative ECL.
Data source location	Zagreb, Croatia
Data accessibility	Data is supplied in this article