Table 3.
Differentially expressed proteins in ΔSsr1—Shigella flexneri.
| Spot no. | NCBI GI identifier | Protein description | Average ratio | pI | Mr | Protein score | Final_Localization |
|---|---|---|---|---|---|---|---|
| DOWN-REGULATED PROTEIN SPOTS IN Δ Ssr1 MUTANT | |||||||
| A04 | gi|56479706 | Leucyl-tRNA synthetase | 1.92316 | 5.11 | 97,815 | 154 | Cytoplasmic |
| A07 | gi|24112341 | Aminopeptidase | 1,000,000 | 5.12 | 99,418 | 250 | Cytoplasmic |
| A09 | gi|24111612 | Outer membrane protein assembly factor YaeT | 1,000,000 | 4.93 | 90,611 | 318 | OuterMembrane |
| A10 | gi|24112901 | Phosphoenolpyruvate synthase | 3.50291 | 4.93 | 87,809 | 222 | Cytoplasmic |
| A14 | gi|24111463 | Molecular chaperone DnaK* | 1.92652 | 4.83 | 69,142 | 201 | Cytoplasmic |
| A19 | gi|24113761 | Phosphoenolpyruvate-protein phosphotransferase | 1.94119 | 4.78 | 63,722 | 172 | Cytoplasmic |
| A25 | gi|56480123 | GTP-binding protein Der | 1,000,000 | 5.52 | 55,089 | 295 | Cytoplasmic |
| A26 | gi|24115037 | ATP synthase F0F1 subunit alpha | 2.13811 | 5.8 | 55,416 | 140 | Cytoplasmic |
| A29 | gi|24115456 | Peptidase PmbA | 1.88864 | 5.48 | 48,624 | 220 | Cytoplasmic |
| A36 | gi|24111974 | Galactokinase | 1.70124 | 5.36 | 41,928 | 384 | Cytoplasmic |
| A37 | gi|24113879 | Bifunctional nitric oxide dioxygenase/dihydropteridine reductase 2 | 2.42852 | 5.49 | 43,998 | 94 | Cytoplasmic |
| A40 | gi|56479896 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH-A) | 2.00224 | 6.61 | 35,681 | 67 | Cytoplasmic |
| A41 | gi|24112338 | Outer membrane protein F | 1.84728 | 4.76 | 39,339 | 316 | OuterMembrane |
| A42 | gi|24113600 | Porin | 2.37934 | 4.56 | 41,377 | 249 | OuterMembrane |
| A43 | gi|56479781 | Outer membrane protein OmpA* | 1.79543 | 5.65 | 37,374 | 273 | OuterMembrane |
| A47 | gi|24114191 | Agmatinase | 3.24396 | 5.14 | 33,764 | 112 | Cytoplasmic |
| A48 | gi|56479821 | DNAse | 1,000,000 | 5.13 | 30,233 | 122 | Cytoplasmic |
| A49 | gi|24115117 | Phospholipase A | 1,000,000 | 5.15 | 33,142 | 184 | OuterMembrane |
| A50 | gi|24113681 | Histidine ABC transporter substrate-binding protein HisJ | 1,000,000 | 6.77 | 29,067 | 176 | Periplasmic |
| A51 | gi|56480449 | Uridine phosphorylase | 1.73923 | 5.81 | 27,341 | 114 | Cytoplasmic |
| A53 | gi|24112822 | Hypothetical protein SF1441 | 2.04377 | 5.5 | 27,813 | 92 | OuterMembrane |
| A55 | gi|24114860 | Glutathione S-transferase | 1,000,000 | 5.10 | 22,762 | 220 | Cytoplasmic |
| A56 | gi|24113642 | Hypothetical protein SF2345 | 1.83302 | 5.29 | 11,280 | 240 | Unknown |
| A58 | gi|24114909 | Deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) | 1.97105 | 5.05 | 16,433 | 381 | Cytoplasmic |
| A59 | gi|24115554 | 30S ribosomal protein S6 | 2.41987 | 5.26 | 15,177 | 81 | Cytoplasmic |
| A60 | gi|24112560 | Cell division topological specificity factor MinE | 2.01343 | 5.15 | 10,286 | 57 | Cytoplasmic |
| UP-REGULATED PROTEIN SPOTS IN Δ Ssr1 MUTANT | |||||||
| B02 | gi|31983589 | Hypothetical protein CP0125 | 7.76534 | 6.13 | 70,080 | 161 | Extracellular |
| B03 | gi|24112624 | Nitrate reductase 1 subunit beta | 8.3085 | 6.36 | 59,012 | 437 | CytoplasmicMembrane |
| B04 | gi|24114695 | Glycogen synthase | 2.9026 | 6.05 | 52,919 | 143 | Cytoplasmic |
| B05 | gi|24113822 | Hypothetical protein SF2538 | 2.71421 | 7.08 | 53,874 | 147 | Unknown |
| B06 | gi|24114874 | L-lactate dehydrogenase | 1,000,000 | 6.33 | 42,902 | 303 | Cytoplasmic |
| B09 | gi|31983568 | Mxi-Spa secretion machinery protein | 4.36788 | 4.82 | 40,280 | 196 | Extracellular |
| B10 | gi|24114573 | DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) | 2.32256 | 4.98 | 36,717 | 417 | Cytoplasmic |
| B16 | gi|31983586 | Hypothetical protein CP0126 | 3.34543 | 5.65 | 36,696 | 169 | Extracellular |
| B19 | gi|24111977 | Phospho-2-dehydro-3-deoxyheptonate aldolase | 2.61593 | 6.14 | 38,399 | 115 | Cytoplasmic |
| B20 | gi|56479896 | Glyceraldehyde-3-phosphate dehydrogenase | 2.05812 | 6.61 | 35,681 | 315 | Cytoplasmic |
| B22 | gi|24113759 | Cysteine synthase A | 1,000,000 | 5.83 | 34,553 | 300 | Cytoplasmic |
| B24 | gi|24112168 | Glycosyl transferase | 2.81361 | 6.08 | 35,303 | 78 | Cytoplasmic |
| B26 | gi|24114611 | FKBP-type peptidylprolyl isomerase | 3.85906 | 8.39 | 28,910 | 206 | Cytoplasmic |
| B29 | gi|56479617 | 30S ribosomal protein S2 | 2.10231 | 6.61 | 26,812 | 232 | Cytoplasmic |
| B37 | gi|24114648 | Phosphoglycolate phosphatase | 1,000,000 | 4.58 | 27,414 | 192 | Cytoplasmic |
| B40 | gi|56480611 | Purine nucleoside phosphorylase | 6.93801 | 5.42 | 26,147 | 383 | Cytoplasmic |
| B41 | gi|24112000 | Succinate dehydrogenase iron-sulfur subunit | 1.96182 | 6.32 | 27,393 | 336 | CytoplasmicMembrane |
| B43 | gi|24112177 | Glutamine ABC transporter ATP-binding protein | 1,000,000 | 6.25 | 26,699 | 90 | CytoplasmicMembrane |
| B48 | gi|24115367 | LexA repressor | 6.58657 | 6.23 | 22,344 | 472 | Cytoplasmic |
| B52 | gi|24114332 | Esterase | 6.63036 | 4.61 | 21,742 | 99 | Cytoplasmic |
| B54 | gi|24115223 | Ribonuclease activity regulator protein RraA | 2.76987 | 4.07 | 17,464 | 109 | Cytoplasmic |
| B57 | gi|56479823 | Hypothetical protein SF1112 (UPF0227 protein YcfP) | 2.99923 | 6.13 | 21,441 | 302 | Cytoplasmic |
| B58 | gi|24113666 | Hypothetical protein SF2370(UPF0304 protein YfbU) | 2.19077 | 5.95 | 19,649 | 255 | Cytoplasmic |
| B61 | gi|31983576 | IpgC, cytoplasmic chaperone for IpaB and IpaC | 5.56608 | 4.58 | 17,916 | 343 | Cytoplasmic |
*
Represent the potential targets of Ssr1, which were predicted by sTarPicker method.