Subject area	Cell Biology and Kinase signaling
More specific subject area	MAP kinase signaling
Type of data	Table and graph
How data was acquired	LC MS/MS, bottom-up approach with CID in the ion trap. Peptides were separated on a Waters nanoACQUITY (column: 75 µm×250 mm eluted at 300 nl/min.; 80 min run) with MS analysis on an Orbitrap Elite mass spectrometer, Thermo Fisher.
Data format	Filtered by Proteome Discoverer v1.4, and database searched via in-house MASCOT Search Engine.
Experimental factors	Effect of MKK3 deletion on the proteome of mouse embryonic fibroblasts
Experimental features	Baseline difference in MKK3 knockout and wild-type mouse embryonic fibroblasts
Data source location	New Haven, CT, USA.
Data accessibility	Data available within this article and at: http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs