Fig. 5.

MSC-like cell secreted factors on quiescent osteosaroma cells in vitro. (a) Representative image of spheres obtained with MNNG-HOS cells cultured under an anchorage-independent condition. Immunohistochemistry detection of Ki-67 revealed a high proportion of proliferating cells (>50%). (b) Relative expression fold changes are presented for mRNA of MNNG-HOS cells cultured either under anchorage (monolayer) or non-anchorage conditions (spheres). Gene name symbols with corresponding full names are indicated in Table 1. Results are means of 3 samples and are presented with standard deviations. Significant differences between the anchorage and non-anchorage culture conditions are indicated (*: p<0.05; ***: p<0.001). (c) Dot plots present the % of MNNG-HOS cells in different cell cycle phases following DNA and Ki-67 detection by flow cytometry. MNNG-HOS cells cultured in oncosphere were incubated for 48 h in medium not supplemented (0% FBS) or supplemented with 10% FBS (10%) or 25% ADSC/MSC-CM. Results are representative of 3 independent experiments.