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. 2016 Jan 28;5(1):22–29. doi: 10.1016/j.jbo.2016.01.002

Fig. 2.

Fig. 2

Hypoxia-induced chemo-resistance is HIF-1-independent in U2-os cells. A–C: Western blot analysis of HIF-1α and HIF-1β in U2-os cells with the pre-incubation in normoxia or hypoxia for 6, 12 or 24 h and the doxorubicin treatment with 20 μM for 2 h, the level of HIF-1α or HIF-1β was relative to β-actin; D–F: Western blot analysis of HIF-1α, cleaved caspase 3 and lyzed PARP, in U2-os cells post the transfection with 20 or 50 nM siRNA- HIF-1α or control siRNA, with β-actin as internal control; G and H: Viability reduction and apoptosis induction of U2-os cells post the transfection with 20 or 50 nM siRNA- HIF-1α or control siRNA, via MTT assay and FACScan flow assay. Each result was averaged for triple independent experiments. **p<0.01, ***p<0.001, ns: no significance.