FIG. 1.

Primary mouse Achilles tenocytes (pMATs) can be isolated from tissue and cultured in vitro. (A) Picture showing harvest of an Achilles tendon from a 4-week-old CD1 mouse. (B) Light microscopy image of pMATs in culture 7 days after isolation from tendons. Black bar denotes 100 μM (C) schematic representation of piggyBac immortalization vector MPH86 showing the SV40 large T antigen (LTA) expression cassette flanked by FLP recombination target (FRT) sites and hygromycin resistance gene. Color images available online at www.liebertpub.com/tec