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. 2016 Mar 1;29(2):112–127. doi: 10.1089/vim.2015.0105

FIG. 4.

FIG. 4.

IFN-γ-producing CD4 and CD8 T-cell and NK-cell responses. MNCs were stimulated with semipurified AttHRV antigen in vitro for 17 h (Brefeldin A was added for the last 5 h to block IFN-γ secretion) and then subjected to intracellular staining and flow cytometry for collecting the frequency data of IFN-γ-producing CD4 (a) and CD8 (b) T cells and NK cells (c). Data are presented as mean frequency ± standard error of the mean (n = 3 or 5). See Figure 3 legend for panel description and statistical analysis.