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. 2016 Mar 1;30(5):502–507. doi: 10.1101/gad.273821.115

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© 2016 Arnes et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — βlinc1 predominantly regulates the expression of β-cell-enriched genes that map to the same chromosomal region as βlinc1. (A) Pie chart showing the total numbers and relative percentages of up-regulated and down-regulated genes (P < 0.05) in βlinc1^+/− versus βlinc1^+/+. (B) Fold change and chromosomal location of the 10 most significantly down-regulated genes in βlinc1^+/− compared with βlinc1^+/+. A large proportion of these genes is in chromosome 2 (Supplemental Fig. 10a). (C) Schematic representation of the mouse karyotype. The red box denotes the location of the dysregulated genes on chromosome 2 in βlinc1^+/− compared with βlinc1^+/+ mice. (D) qRT–PCR validation of RNA-seq results for the genes located in chromosome 2. (*) P < 0.05 versus βlinc1^+/+; (#) P < 0.05 versus βlinc1^+/−. (E) Down-regulation of βlinc1 with two different siRNAs in MIN6 cells recapitulates the increase in somatostatin expression and down-regulation of Scg5 and Pax6 seen in vivo. n = 4. Error bars represent ±SEM. (*) P < 0.05 versus scrambled. Student's t-test.