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. 2016 Mar 1;30(5):522–534. doi: 10.1101/gad.271890.115

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© 2016 Yan et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — p53 expression was suppressed by PCBP4 deficiency via ZFP871. (A) The levels of p53 and p21 proteins were measured in C2C12 cells transfected with pcDNA3 or pcDNA3-HA-ZFP871 in the absence or presence of 0.35 µg/mL doxorubicin for 24 h. Actin was used as a loading control. (B) The levels of p53 and p21 proteins were measured in C2C12 cells transfected with two unique scrambled siRNAs or siRNAs against ZFP871. (C,D) The levels of p53 and p21 proteins were measured in murine FL83B (C) and TS20 (D) cells transfected with a scrambled siRNA (Scr-1) or a siRNA against ZFP871 (siZFP871-1) in the absence or presence of 0.35 µg/mL doxorubicin for 24 h. (E) The levels of p53 and PCBP4 proteins were measured in C2C12 cells transiently transfected with a scrambled siRNA (Scr-1), siRNA against PCBP4 (siPCBP4), siRNA against ZFP871 (siZFP871-1), or both siPCBP4 and siZFP871 for 3 d. (F) The level of ZFP871 transcript was measured by qRT–PCR in C2C12 cells treated as in E.