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. 2016 Mar 3;7:10857. doi: 10.1038/ncomms10857

Figure 4. IL-2 rescues Th9 differentiation in Itk−/− CD4+ T cells.

Figure 4

(a-c) Sorted naïve CD4+ T cells from WT and Itk−/− mice were differentiated under Th9 conditions plus TL1A for 3 days and pSTAT5 (a) and CD25 (b) were determined by flow cytometry: WT (black), Itk−/− (grey) lines. (c) Sorted naive CD4+ T cells were differentiated as in a or under Th17 conditions and IL-2 in supernatants were determined at 48 and 72 h by Luminex. Th9 conditions: black bars. Th17 conditions: hatched bars. (d,e) Sorted naïve CD4+ T cells were differentiated for 3 days under Th9 (d) or Th9 plus TL1A (e) conditions in absence or presence of blocking anti-IL-2 plus hIL-2, restimulated with PMA and Ionomycin and IL-9 analysed by flow cytometry. (f) Sorted naive CD4+ T cells from WT and Itk−/− mice were stained with CSFE, differentiated, and restimulated with PMA and Ionomycin to evaluate IL-9 expression. Data in a-f are representative of one out of at least three independent experiments.