Figure 9. Itk-deficient mice have late defects in ILC2 function.

(a) Outline of 14day papain-induced sensitization. (b) Numbers of ILC2⁺, IL-9- and IL-13-ILC2⁺ producing cells in lungs are shown from cells harvested at day 14 from female mice challenged with papain. (c) Outline of papain sensitization of female mice for 4 days. (d) Numbers of ILC2⁺ and IL-9-producing ILC2⁺ cells from mice treated with papain on day 4. (e) Outline of IL-33-induced sensitization for 4 days. (f) Numbers of ILC2⁺ cells in lungs harvested at day 4 from mice challenged with IL-33. (g) ILC2⁺ cells were sorted from IL-33-treated mice, cultured with 10 μg ml⁻¹ of the indicated cytokines for 3 days and IL-9, IL-5 and IL-13 was measured in the culture supernatants (IL-9 and IL-5 have the same scale for Y axis). Experiments were performed at least twice using six female mice for papain-treated or IL-33 groups and four mice for PBS controls. (b,d,f) Mean±s.e.m. from one representative experiment, *P<0.05, **P<0.01 using Mann–Whitney test.