Table 1.

Primers for real-time quantitative PCR analysis

Microbial species	Primer sequence (5′-3′)	Product size (bp)	Reference
Total bacteria	FP: CGGCAACGAGCGCAACCC RP: CCATTGTAGCACGTGTGTAGCC	130	Zeng et al., 2012
Ruminococcus flavefaciens	FP: CGAACGGAGATAATTTGAGTTTACTTAGG RP: CGGTCTCTGTATGTTATGAGGTATTACC	132	Zeng et al., 2012
Fibrobacter succinogenes	FP: GTTCGGAATTACTGGGCGTAA A RP: CGCCTGCCCCTGAACTATC	121	Zeng et al., 2012
Ruminococcus albus	FP: CCCTAAAAGCAGTCTTAGTTCG RP: CCTCCTTGCGGTTAGAACA	176	Zeng et al., 2012
Butyrivibrio fibrisolvens	FP: GAGGAAGTAAAAGTCGTAACAAGGTTTC RP: CAAATTCACAAAGGGTAGGATGATT	160	Zeng et al., 2012
Methanogen	FP: GGATTAGATACCCSGGTAGT RP: GTTGARTCCAATTAAACCGCA	174	Christophersen, 2007
Fungus	FP: GAGGAAGTAAAAGTCGTAACAAGGTTTC RP: CAAATTCACAAAGGGTAGGATGATT	120	Denman and McSweeney, 2006
Protozoa	FP: GCTTTCGWTGGTAGTGTATT RP: ACTTGCCCTCYAATCGTWCT	223	Sylvester et al., 2004

PCR, polymerase chain reaction.