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. 2016 Mar 8;6:22775. doi: 10.1038/srep22775

Figure 2.

Figure 2

(A) Hypoxia induces dynamic changes in the mRNA and protein expression profile of the HIF1A in HUVECs. The mRNA levels were monitored in qRT-PCR experiments. The results from 3 independent experiments (n = 12) are plotted normalized to TBP mRNA levels and expressed as a fold-change over the normoxic control. Error bars represent standard deviations. (B) Hypoxia induces dynamic changes of protein levels of HIF-1α and (C) the bar graphs show the relative protein amounts at each time point. The protein levels of were detected with SDS-PAGE and Western Blot and related to total protein levels. 2 individual samples (4 μg of total protein per lane) were tested for each time point and the experiments were repeated twice. The protein levels (bar graphs) are normalized to the normoxic control. Significant changes (p < 0.05) are marked with an “*”. (D) The predicted target site of miR-429 in HIF3A2-3. The localization of putative miR-429 sequence is shown aligned with the 3′UTR of HIF3A isoforms. The numbering is based on the human NCBI genomic sequence (RefSeq). (E) Hypoxia-induced changes in the expression profile of miR-429 in HUVECs. The miRNA level was monitored in quantitative real-time PCR experiments. The results from 3 independent experiments (n = 18) are plotted normalized to RNU44 and expressed as a fold change over the normoxic control (*P < 0.05).