Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 8;6:22775. doi: 10.1038/srep22775

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Hypoxia induces dynamic changes in expression profiles of DDIT4 (A) in HUVECs. The results from 3 independent experiments (n = 18) are plotted normalized to TBP mRNA levels and expressed as a fold-change over the normoxic control. Error bars represent standard deviations. miR-429 mimic and antagomir modulate DDIT4 expression. (B) HUVECs were transfected with miR-429 mimic or antagomir and the mRNA levels were monitored in qRT-PCR experiments in normoxic conditions and after 4 and 8 hours of hypoxia. The mRNA levels from 3 independent experiments (n = 12) were normalized TBP mRNA levels and expressed as a fold change over the transfection control. miR-429 indirectly modulates the expression of DDIT4 mRNA levels during hypoxia through its actions on HIF3A mRNA. (C) mRNA levels were monitored in qRT-PCR experiments following transfection of HUVECs with HIF3A TP and HIF1A TP together with miR-429 analog (Mimic 429). The mRNA levels results from 3 independent experiments (n = 12) are normalized to TBP mRNA levels and expressed as a fold-change over the transfection control. Significant changes (p < 0.05) are marked with an “^*”.