Skip to main content
. 2016 Mar 8;6:22775. doi: 10.1038/srep22775

Figure 7.

Figure 7

(A) Model of the miR-429 regulatory effects on the HIF-1/HIF-3 transition in HUVECs during hypoxia. During normoxia, miR-429 controls steady-state levels of HIF1A and HIF3A mRNA and keeps the levels low. Protein levels remain low through posttranslational modifications and subsequent degradation of these transcription factors when the oxygen levels are normal. During the early stages of hypoxia, HIF1A message and protein are dramatically elevated, while the HIF3A variants and protein remain low. HIF-1α drives a number of pro-survival and angiogenic target genes and also promotes miR-429 and HIF3A up regulation. miR-429 decreases the steady-state levels of both HIF1A and HIF3A mRNA. During prolonged hypoxia, HIF-1α levels and miR-429 are low, and HIF3A mRNA and HIF-3α protein accumulate over time, leading to the induction of HIF-3 pro-survival gene expression. (B) The HIF1A and HIF3A relative expression levels (Fragments Per Kilobase of transcript per Million mapped reads) in HUVECs, analysis made base on 2 NGS analysis of 2 independent samples of normoxic HUVEC cells passage 3. Error bars represent SEM.