Table S1.

Total sample size and distribution for analysis of wild type growth cone collapse

Treatment	Growth cones (n)	Collapsed gc (n)	Collapsed gc (%)	Dishes (n)	Mean collapse	SEM	Explants (n)	Diff expts (n)
Control	2970	265	8.9%	14	9.4%	0.9%	101	11
0.1 nM LPA	746	229	30.7%	5	31.7%	7.0%	33	4
1 nM LPA	2189	840	38.4%	14	42.2%	5.0%	102	11
10 nM LPA	1304	1157	88.7%	13	89.4%	1.3%	94	10
100 nM LPA	762	744	97.6%	13	98.2%	0.5%	77	10
1 μM LPA	418	415	99.3%	6	98.8%	0.7%	34	5
10 μM LPA	532	528	99.2%	5	99.4%	0.4%	39	4
1 nM S1P	501	106	21.2%	3	21.0%	1.2%	24	3
10 nM S1P	366	66	18.0%	3	17.0%	1.5%	22	3
100 nM S1P	527	89	16.9%	3	16.3%	2.3%	25	3
1 μM S1P	900	151	16.8%	6	16.3%	2.5%	43	5

Notes: Mean collapse rate and SEM is per dish. “Diff expts” indicates independent experiments done on different days, in which each experiment included one or more animals whose retinal explants were pooled for that experiment.

Abbreviations: LPA, lysophosphatidic acid; S1P, sphingosine 1-phosphate; SEM, standard error of mean.