Table S2.

Total sample size and distribution for analysis of growth cone collapse from LPA receptor mutant mouse retinal explants

Genotype	Treatment	Growth cones (n)	Collapsed gc (n)	Collapsed gc (%)	Explants (n)	Animals (n)
Wild type	Control	798	67	8%	23	3
Wild type	1 nM LPA	785	217	28%	21	3
Wild type	10 nM LPA	449	386	86%	23	3
Wild type	100 nM LPA	233	227	97%	19	3
Lpar2	Control	877	85	10%	30	5
Lpar2	1 nM LPA	986	244	25%	35	5
Lpar2	10 nM LPA	671	554	83%	34	5
Lpar2	100 nM LPA	483	444	92%	31	5
Lpar1 Lpar2	Control	435	41	9%	16	2
Lpar1 Lpar2	1 nM LPA	404	107	26%	17	2
Lpar1 Lpar2	10 nM LPA	275	208	76%	14	2
Lpar1 Lpar2	100 nM LPA	137	130	95%	14	2
Lpar2 Lpar3	Control	424	49	12%	13	2
Lpar2 Lpar3	1 nM LPA	372	114	31%	16	2
Lpar2 Lpar3	10 nM LPA	355	306	86%	16	2
Lpar2 Lpar3	100 nM LPA	179	174	97%	14	2
Lpar1 Lpar3	Control	151	7	5%	5	1
Lpar1 Lpar3	1 nM LPA	199	65	33%	7	1
Lpar1 Lpar3	10 nM LPA	92	71	77%	9	1
Lpar1 Lpar3	100 nM LPA	36	32	89%	4	1
Lpar1 Lpar2 Lpar3	Control	276	12	4%	9	1
Lpar1 Lpar2 Lpar3	1 nM LPA	147	22	15%	8	1
Lpar1 Lpar2 Lpar3	10 nM LPA	351	313	89%	9	1
Lpar1 Lpar2 Lpar3	100 nM LPA	104	91	88%	8	1

Notes: For each genotype, multiple growth cones from multiple explants were analyzed. In most experiments, explants were obtained from multiple animals and analyzed in separate dishes. The wild type animals presented here are littermates of the Lpar2 animals. Control had no LPA addition.

Abbreviations: LPA, lysophosphatidic acid; gc, growth cone.