Table S3.

Sample size and distribution for neurite retraction assay

Genotype	Treatment	Explants (n)	Mean retraction	SEM	Dishes (n)	Animals (n)
Wild type	Control	12	−8.7%	6.1%	5	4*
Wild type	1 nM LPA	17	47.5%	4.2%	6	5*
Wild type	10 nM LPA	20	80.1%	5.9%	9	6*
Wild type	100 nM LPA	25	97.2%	0.7%	9	7*
Lpar2	10 nM LPA	13	81.1%	3.3%	5	4
Lpar1 Lpar2	10 nM LPA	21	78.4%	4.2%	7	6
Lpar1 Lpar2 Lpar3	10 nM LPA	3	63.4%	22.9%	1	1

Note: Total number of explants analyzed (n) for each condition along with the mean retraction (%), where negative retraction would be growth, and SEM. In addition, the total number of dishes analyzed as well as the number of animals analyzed for each condition is indicated.

^*For wild type analysis, each experiment represented one or more animals whose retinas were removed and explants pooled for analysis; thus, the number of animals indicated is the number of independent experiments, which is a minimum number of animals.

Abbreviations: LPA, lysophosphatidic acid; SEM, standard error of mean.