. 2016 Mar 8;7:273. doi: 10.3389/fpls.2016.00273

Table 5.

Unconditional additive and interacting effects of QTL × water environment of identified QTLs for the greenness of flag leaf.

*QTL*	Flanking markers	Stage^a	A^b	AE^c	*H²(A)^b(%)*	*H²(AE)^c(%)*
Qspad.acs-1A.1	Xgwm633-Xgwm164	S1	0.81^***		0.73
		S2	0.72^***		0.85
Qspad.acs-1A.2	Xgwm135-Xwmc304	S1	0.76^***		0.91
		S2	0.65^***		0.63
		S3	0.48^***	−0.66^*(E2_DS), −0.72^(E3_DS), 0.78^**(E2_WW)	0.43	2.24
Qspad.acs-1A.3	Xgwm357-Xgwm633	S2	0.72^***	−0.85^***(E1_WW)	0.87	2.54
Qspad.acs-1B.1	Xgwm11-Xwmc626	S1	−0.80^***		0.63
		S2	−1.10^***	0.98^*(E1_DS), −0.92^*(E3_WW)	0.92	3.24
		S3	−0.76^***		1.05
		S4	−0.41^***		1.31
Qspad.acs-1B.2	Xmag2064-Xwmc694	S2	−0.76^***	0.88^*(E1_DS), 0.83^(E3_DS), −0.75^**(E2_WW)	0.82	1.64
Qspad.acs-1B.3	Xwmc85-Xcfd65	S2	−0.67^***		0.68
		S3	−0.45^***		0.84
Qspad.acs-1B.4	Xwmc830-Xwmc44	S4	0.59^***	−0.31^**(E1_DS)	0.83	1.34
Qspad.acs-1B.5	Xwmc582-Xgwm374	S1	−0.59^***		0.56
Qspad.acs-2A.1	Xgwm339-Xgwm95	S1	0.77^***	−0.93^*(E2_WW), −0.99^*(E3_WW)	1.05	1.71
		S3	0.46^***	−0.56^*** (E1_DS)	0.69	0.87
Qspad.acs-2A.2	Xgwm249-Xcfa2263	S1	−0.57^***		0.59
Qspad.acs-2A.3	Xgwm558-Xbarc208	S1	0.94^***	0.34^**(E2_WW)	1.06	1.14
		S2	0.84^***	1.12^*(E2_DS), −1.03^*(E2_WW)	1.16	5.42
		S3	0.70^***		1.80
		S4	0.42^***	−0.49^*(E1_DS), 0.40^(E2_WW), 0.49^**(E3_WW)	0.92	1.91
Qspad.acs-2A.4	Xmag2150-Xgwm339	S2	0.75^***		0.95
Qspad.acs-2A.5	Xwmc296-Xgwm122	S4	0.49^***	−0.56^*(E1_DS), 0.29^ (E2_WW)	0.84	1.54
		S5	0.28^*	−0.23^* (E3_DS)	0.64	2.74
Qspad.acs-2A.6	Xwmc644-Xmag1730	S1	0.93^***		1.29
Qspad.acs-2B.1	Xcfa2278-Xgwm55	S2	0.64^***		0.58
Qspad.acs-2B.2	Xgwm55-Xbarc128	S2	0.78^***		0.77
		S3	0.61^***	0.50^***(E2_WW)	0.72	2.09
Qspad.acs-2B.3	Xgwm388-Xmag3319	S3	0.65^***		0.59
		S4	0.49^***		0.55
Qspad.acs-2B.4	Xwmc223-Xbarc101	S2	0.83^***		0.78
Qspad.acs-3A.1	Xwmc11-Xgwm391	S2	−0.67^***	0.78^***(E2_DS)	0.76	1.56
Qspad.acs-3A.2	Xwmc532-Xgwm674	S3	−0.55^***	0.65^* (E3_DS), −0.66^* (E2_WW), −0.59^*** (E3_WW)	0.52	2.15
		S4	−0.41^***		0.40
Qspad.acs-3A.3	Xcfa2234-Xwmc695	S4	0.50^***		0.71
Qspad.acs-3B.1	Xwmc808-Xbarc102	S3	0.58^***	−0.41^*(E1_DS), −0.45^(E2_DS), 0.47^**(E1_WW)	0.42	1.66
		S4	0.44^***		0.63
Qspad.acs-3B.2	Xmag3356-Xwmc291	S3	0.61^***		0.75
Qspad.acs-3B.3	Xwmc540-Xgwm566	S1	−0.64^***		0.75
Qspad.acs-4A.1	Xmag987-Xbarc78	S5	−0.32^**	−0.43^* (E2_DS), −0.41^* (E3_DS), 0.30^** (E1_WW)	0.61	2.39
Qspad.acs-4B.1	Xgwm513-Xbarc1142	S4	−0.47^***	−0.55^**(E1_DS), 0.25^ (E3_WW)	0.37	1.58
Qspad.acs-4B.2	Xgwm540-Xcfd2	S4	0.62^***		0.50
Qspad.acs-4B.3	Xgwm149-Xgwm495	S3	0.48^***		0.38
Qspad.acs-5B.1	Xmag532-Xgwm499	S2	0.51^***		0.44
Qspad.acs-5B.2	Xwmc734-Xwmc235	S1	−0.88^***	−0.25^*(E3_WW)	0.86	0.89
		S2	−0.76^***		0.86
		S3	−0.64^***		1.11
		S4	−0.49^**	−0.52^* (E1_DS), 0.30^(E3_WW)	0.90	1.76
Qspad.acs-5B.3	Xwmc235-Xcfd10	S3	−0.67^***		0.89
Qspad.acs-5B.4	Xwmc415-Xwmc508	S3	0.45^***		0.69
Qspad.acs-5D.1	Xwmc161-Xgwm565	S4	−0.55^***	−0.62^* (E2_DS), −0.58^* (E3_DS), 0.31^** (E1_WW)	0.36	2.05
Qspad.acs-5D.2	Xbarc205-Xgwm232	S5	0.33^**	−0.39^*** (E2_DS), 0.22^*(E1_WW)	0.47	1.91
Qspad.acs-6A.1	Xwmc201-Xwmc684	S2	0.35^**	−0.39^*(E2_WW), 0.45^*(E3_WW)	0.49	1.56
Qspad.acs-6A.2	Xbarc171-Xgwm427	S2	−0.59^***	0.28^(E2_WW), 0.35^*(E3_WW)	0.28	1.26
		S3	−0.40^***		0.36
		S4	−0.39^***		0.24
Qspad.acs-6A.3	Xgwm169-Xwmc580	S3	−0.72^***	−0.30^(E1_DS), −0.33^(E3_DS)	0.60	1.14
Qspad.acs-6A.4	Xbarc113-Xwmc621	S4	−0.69^***	−0.72^*(E2_DS), −0.76^(E3_DS), 0.39^**(E3_WW)	0.56	2.49
Qspad.acs-6B.1	Xgwm508-Xmag1378	S1	0.62^***		0.70
		S3	0.44^***		0.38
Qspad.acs-6B.2	Xcfd13-Xwmc737	S4	0.42^***		0.28
Qspad.acs-6B.3	Xmag3469-Xgwm644	S3	−0.51^***		0.32
Qspad.acs-6B.4	Xgwm644-Xmag1266	S3	−0.40^***		0.25
Qspad.acs-7A.1	Xwmc603-Xwmc116	S3	0.59^***	0.73^*(E2_DS), 0.81^(E3_DS), −0.72^**(E2_WW)	0.56	2.26
Qspad.acs-7A.2	Xwmc273-Xwmc83	S4	−0.45^***		0.26
Qspad.acs-7B.1	Xgwm302-Xbarc258	S3	−0.57^***	−0.79^*(E1_DS), −0.72^*(E3_DS)	0.31	2.32
		S5	−0.39^***		0.36
Qspad.acs-7B.2	Xwmc517-Xbarc315	S3	−0.73^***	−0.26^(E3_DS), 0.29^*(E3_WW)	0.60	0.94
Qspad.acs-7B.3	Xwmc311-Xgwm611	S4	−0.62^***		0.55
Qspad.acs-7D.1	Xgwm428-Xbarc111	S3	−0.66^***	−0.83^*(E2_DS), −0.80^(E3_DS), 0. 85^**(E1_WW)	0.54	2.67
Qspad.acs-7D.2	Xgwm37-Xwmc634	S4	−0.43^***	−0.53^*(E1_DS), −0.50^(E2_DS), 0.51^**(E2_WW)	0.25	1.80
		S5	−0.26^*	−0.30^***(E3_DS)	0.54	1.56
Qspad.acs-7D.3	Xcfd46-Xwmc438	S3	−0.73^***	−0.87^*(E1_DS), −0.80^*(E2_DS)	0.68	2.52

1–S5 are as shown in Table 1.

A, the additive effect. A positive value indicates the genetic effect from Q9086 allele, and a negative value represents the genetic effect from Longjian 19 allele;

P ≤ 0.01,

^**

P ≤ 0.005, and

^***

P ≤ 0.001; H²(A) (%) indicates the proportion of phenotypic variance explained by additive QTL.

AE, the additive QTL × environment interaction effects in drought stress (DS) and the well-watered (WW) conditions in E1–E3 shown in Table 1. H²(AE)(%) indicates the phenotypic variance explained by additive QTL × environment interaction.