Table 2.
Peptides selected to cover the sequence of the coat protein (CP) showing the percentage exchange and the difference of exchange between two repeat measurements monitoring HDX-MS of CP2: TR
| Peptide | Exchange measured |
Difference between repeat measurements |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time of exchange (seconds) | ||||||||||||||
| 30 | 100 | 300 | 1000 | 3000 | 10000 | 30000 | 30 | 100 | 300 | 1000 | 3000 | 10000 | 30000 | |
| [1–4] | 25.9% | 23.6% | 22.8% | 22.4% | 23.0% | 23.8% | 23.4% | 4.5% | 3.2% | 0.4% | 1.3% | 1.0% | 0.5% | 0.1% |
| [5–7] | 1.7% | −1.3% | 1.8% | 15.4% | 22.4% | 20.4% | 21.5% | 8.7% | 0.1% | 0.7% | 0.5% | 1.8% | 0.9% | 0.9% |
| [8–24] | 29.2% | 29.5% | 30.6% | 31.8% | 36.0% | 40.4% | 45.1% | 1.1% | 1.1% | 1.2% | 0.3% | 1.1% | 1.3% | 0.7% |
| [25–31] | 34.0% | 35.2% | 36.1% | 37.9% | 45.1% | 52.3% | 57.6% | 7.4% | 0.2% | 0.1% | 0.4% | 2.8% | 0.4% | 0.2% |
| [32–41] | 58.1% | 62.1% | 65.3% | 66.0% | 67.5% | 66.2% | 67.9% | 2.5% | 1.0% | 1.0% | 0.3% | 2.3% | 3.8% | 0.8% |
| [42–48] | 17.7% | 18.5% | 19.1% | 17.5% | 17.6% | 19.7% | 21.0% | 4.8% | 1.7% | 2.5% | 0.5% | 0.8% | 2.5% | 1.4% |
| [49–74] | 38.4% | 39.0% | 40.5% | 41.5% | 42.8% | 43.8% | 43.9% | 0.4% | 0.0% | 0.1% | 0.6% | 1.0% | 1.6% | 1.1% |
| [75–81] | 61.7% | 67.8% | 67.2% | 66.2% | 66.9% | 66.6% | 65.5% | 11.9% | 1.9% | 0.8% | 1.6% | 2.7% | 1.2% | 2.1% |
| [82–88] | 5.8% | 5.0% | 5.5% | 7.0% | 11.8% | 20.7% | 27.2% | 4.4% | 1.6% | 0.7% | 1.0% | 0.7% | 1.9% | 0.2% |
| [89–102] | 20.9% | 27.1% | 28.1% | 29.6% | 29.2% | 31.7% | 32.3% | 2.9% | 1.1% | 1.0% | 0.7% | 2.9% | 0.3% | 1.1% |
| [103–129] | 23.9% | 24.4% | 25.9% | 27.4% | 31.0% | 35.2% | 39.0% | 3.2% | 0.3% | 0.9% | 0.2% | 0.9% | 1.9% | 0.3% |
| [104–121] | 11.9% | 13.0% | 12.8% | 12.9% | 14.8% | 18.7% | 23.8% | 1.1% | 0.2% | 0.4% | 0.6% | 0.8% | 0.5% | 0.6% |
| [122–129] | 46.1% | 53.1% | 60.4% | 64.2% | 68.6% | 69.4% | 72.4% | 3.8% | 4.4% | 1.0% | 2.4% | 0.0% | 2.1% | 1.7% |