The maternal mitotypes of feral honey bee colonies at the Welder Wildlife Refuge, San Patricio County, TX, from 1991 to 2001, and again in 2013. Gray bars correspond to colonies with mitochondrial DNA belonging to Apis mellifera lamarckii, white bars correspond to cavities occupied by colonies of eastern European maternal descent, diagonal lines represent cavities occupied by colonies of western European maternal descent, and black bars represent the number of cavities occupied by colonies of A. m. scutellata descent. The percent of tree active cavities occupied by colonies of A. m. scutellata descent over time is represented by the dotted line with white circles (secondary y‐axis), with a broken line between 2001 and 2013 to show that no data were collected during that 12‐year span. Data from 1991 to 2001 are the sum of all established and newly found tree cavities occupied by colonies, as reported in Table 1 by Pinto et al. (2004).