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. 2016 Mar 8;23:32. doi: 10.1186/s12929-016-0249-0

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© Jung et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — EA preconditioning down-regulated the expression of NOX4, not NOX2, after focal cerebral ischemia. a, b Real-time PCR shows the mRNA levels of NOX2 and NOX4 in the ischemic brain tissues 24 h after focal cerebral ischemia. (N = 4, ** P < 0.01 compared with the vehicle group). c Western blots of NOX2 and NOX4 in the ischemic cortex 24 h after focal cerebral ischemia. β-Actin was used as an internal control. d Densitometric quantification graph of the western blot bands of NOX4. Data are expressed as the means ± SEM (N = 4). *P < 0.05 when compared with the vehicle group (Veh)