Fig. 6.

Bit1-shRNA triggered the decreases of Bit1, Bcl-2 and MMP-2 expressions as well as cell apoptosis in xenografted nude mice. When the measurement of tumor volume was terminated, tumor tissues were harvested, and real-time qPCR and Western blotting were utilized to detect the expressions of Bit1, Bcl-2 and MMP-2 mRNAs and proteins. a Real-time qPCR investigation for expressions of Bit1, Bcl-2 and MMP-2 mRNAs in different doses Bit1 shRNA or negative shRNA groups. b Immunohistochemistry assay for expressions of Bit1, Bcl-2 and MMP-2 proteins in different doses Bit1 shRNA or negative shRNA groups. c Average density assay of Bit1, Bcl-2 and MMP-2 protein levels in different doses Bit1 shRNA or negative shRNA groups. d TUNEL assay for cell apoptosis in different doses Bit1 shRNA or negative shRNA groups. e Apoptotic index was counted according to apoptotic cell number under 5 randomly selected fields. *P < 0.05, compared with 5 μg negative group, **P < 0.01, compared with 10 μg negative group