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. 2016 Mar 8;16:62. doi: 10.1186/s12870-016-0750-3

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© Zhang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — HPLC chromatography of SjGMD enzyme assays. a the separation of substrate GDP-Mannose; b ~ d, the separation of enzyme assay after 0, 5 and 30 min, respectively. ①, NAD⁺; ② and ④, unknown impurities from NAD⁺; ③, NADH; ⑤, GDP-Mannose; ⑥, GDP-Mannuronic acid