. 2016 Mar 7;16:189. doi: 10.1186/s12885-016-2180-x

Table 1.

Sequence of primers used for quantitative real-time PCR

Gene	5′-3′ Sequence
TNF-α	Forward: CCCTCACACTCAGATCATCTTCT
TNF-α	Reverse: GCTACGACGTGGGCTACAG
IL-1α	Forward: CGAAGACTACAGTTCTGCCATT
IL-1α	Reverse: GACGTTTCAGAGGTTCTCAGAG
IL-6	Forward: TAGTCCTTCCTACCCCAATTTCC
IL-6	Reverse: TTGGTCCTTAGCCACTCCTTC
CXCL9/MIG	Forward: GGAGTTCGAGGAACCCTAGTG
CXCL9/MIG	Reverse: GGGATTTGTAGTGGATCGTGC
CXCL10/IP-10	Forward: CCAAGTGCTGCCGTCATTTTC
CXCL10/IP-10	Reverse: GGCTCGCAGGGATGATTTCAA
CCL5/RANTES	Forward: GCTGCTTTGCCTACCTCTCC
CCL5/RANTES	Reverse: TCGAGTGACAAACACGACTGC
IL-1β	Forward: GCAACTGTTCCTGAACTCAACT
IL-1β	Reverse: ATCTTTTGGGGTCCGTCAACT
Ocln	Forward: TTGAAAGTCCACCTCCTTACAGA
Ocln	Reverse: CCGGATAAAAAGAGTACGCTGG
ZO-1 (TJP1)	Forward: GCCGCTAAGAGCACAGCAA
ZO-1 (TJP1)	Reverse: TCCCCACTCTGAAAATGAGGA
Cldn3	Forward: ACCAACTGCGTACAAGACGAG
Cldn3	Reverse: CAGAGCCGCCAACAGGAAA
GAPDH	Forward: CTGCACCACCAACTGCTTAG
GAPDH	Reverse: GGGCCATCCACAGTCTTCT