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. 2016 Mar 8;9:24. doi: 10.1186/s13041-016-0207-5

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© Lee et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Wnt3a upregulates NeuroD1 and Ins2 in the mouse hypothalamus. The basal mRNA levels of Ins2 and Wnt3a were measured by qRT-PCR in hypothalamic tissues from C57BL/6 male mice (n = 6−9) (a). C57BL/6 male mice received icv injections of vehicle or Wnt3a (4 or 20 ng), and hypothalamic tissues were dissected after 24 h. The mRNA levels of Ins2 (b) and NeuroD1 (c) were measured by qRT-PCR (n = 6−9). Data are means + SEM. ###p < 0.001 compared with Ins2 mRNA, ***p < 0.001 compared with vehicle administration