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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Bioorg Med Chem. 2015 Feb 13;23(7):1507–1514. doi: 10.1016/j.bmc.2015.02.003

Figure 1.

Figure 1

Effects of curcumin derivatives on EGFR in gefitinib-insensitive cells. The gefitinib-insensitive lung adenocarcinoma cell lines were pre-treated with 10 μM compound, 1–37 as indicated, for 1 h prior to stimulation for 30 min with 20 ng/mL EGF. Cell lysates from (A) CL1-5 carrying wild-type EGFR, or (B) H1975 carrying L858R and T790M double mutations on EGFR, were analyzed by Western blot using antibodies to Tyr1068-phosphorylated EGFR (pEGFR), pan-EGFR (EGFR), and β-actin as a loading control.