Table 1.
Details of oligonucleotide primers used in the current study for RT-LAMP amplification of the FMD viruses 3D polymerase gene. Genome position is arranged according to the FMD virus strain O UKG 35/2001 (GenBank Accession No. AJ539141)
| Virus serotype | Primers | Sequence (5´-3´) | Target (genome position) |
|---|---|---|---|
| O, A, Asia-1 | F | GGAACTGGGTTTTACAAACCTG | 1183-1382 (199 bp) Current study |
| B | CGCAGGTAAAGTGATCTGTAGC | ||
| FIP | CTGCCACGGAGATCAACTTCTCCTGGATCCGACCCTCGAGGCTATCCTCT | ||
| BIP | CTCGCCGTCCACTCTGGACCTGGATCCTGGAATCTCAAAGAGGCCCTG | ||
| FLP | GTATGGTCCCACGGCGTGC | ||
| BLP | GAGTACCGGCGTCTCTTTGAGC | ||
| O | F3 | CATCCTCACCACCCGTAAC | 66-297 (232 bp) Madhanmohan et al. (2013) |
| B3 | GACACCTTTGTGGTCGGTC | ||
| FIP | GGAAGTGTTCGGTCCGCTCACTTTTCCCAGTCAAGCGTTGGAG | ||
| BIP | CAGAGTTGTGCAGGCAGAACGGTTTTAACGTCCGAATGAGTCACTG | ||
| FLP | GGAGTCACATACGGGTACG | ||
| BLP | CACCTCTTCGACTGGGTC | ||
| A | F3 | CTACACTGCGCCTAACCG | 1728-1936 (209 bp) Madhanmohan et al. (2013) |
| B3 | TGGGGCAGTAGAGTTCGG | ||
| FIP | TGCGACTGCCCCTAGGTCACTTTTTAACAGTGTACAACGGGACG | ||
| BIP | GCCCAACTTCCTGCCTCTTTCATTTTCTTCATGCGCACAAGAAGC | ||
| FLP | CAAGTACTCCGCGGCCAGTG | ||
| BLP | GGTGCAATCAAGGCTGACG | ||
| Asia-1 | F3 | CCCCACTGAACACAAAGGC | 287-483 (187 bp) Madhanmohan et al. (2013) |
| B3 | GTGGGGAAAGAGAGTCAGC | ||
| FIP | AGTCACCTCTACGTCCCATCCATTTTGTGTACGGCAGTCTCATGG | ||
| BIP | TTGGAAACCAATTCAACGGCGGTTTTGTCAAGGCTCTTCAGCTCTG | ||
| FLP | CTCGTACGCCTACATGAGGAA | ||
| BLP | GCCTCCTTGTCGCACTTGTG |
F: Forward outer primer, B: Backward outer primer, FIP: Forward inner primer, BIP: Backward inner primer, FLP: Forward loop primer, and BLP: Backward loop primer