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. 2016 Feb 29;4:e1694. doi: 10.7717/peerj.1694

Table 5. Non-parametric tests for all the species.

Results of the non-parametric tests (Spearma’s ρ and Mann–Whitney’s U) conducted separately to each species to test the correlation between the genetic diversity indices (h, π and MNPD) and the independent variables “drainage area,” “inter-basin connectivity,” “hydrological regime” and “latitude.” All tests were bilateral and p-values were Bonferrori corrected for multiple comparisons. Significant p-values (p < 0.005) are highlighted in bold and the direction of the Mann–Whitney’s U tests was indicated: the group (unconnected vs. connected, temporary vs permanent, and southern vs northern) with the highest median was shaded in grey. Analyses were not conducted for species with less than 5 populations (marked with a “*” symbol). In some cases (marked as “constant”), all the populations of a species showed the same values for a given categorical variable (e.g., all were classified as southern regarding “latitude”).

Drainage area (DA) Inter-basin connectivity (IBC) Hydrological regime (HR) Latitude (L)
Species (Nb. populations) Spearman ρ p U p U p U p
Unconnected Connected Temporary Permanent Southern Northern
Dependent variable: h
L. bocagei (N = 39) 0.040 0.808 128 0.578 168 0.988 97 0.008
A. oligolepis (N = 26) −0.169 0.409 88 0.698 34 1.00 35 0.560
P. duriense (N = 14) 0.465 0.094 29 0.240 constant constant
P. polylepis (N = 16) 0.257 0.337 constant 20 0.441 2 0.002
P. willkommii (N = 6) −0.543 0.266 constant constant constant
S. carolitertii (N = 21) 0.114 0.623 69 0.149 13.5 0.667 13.5 0.667
S. pyrenaicus (N = 18) 0.326 0.186 55.5 0.019 51 0.075 constant
S. torgalensis (N = 1) * * * *
S. aradensis (N = 5) 0.447 0.450 constant constant constant
L. comizo (N = 6) 0.395 0.439 constant 1 0.667 constant
L. sclateri (N = 9) 0.402 0.284 12 0.730 constant constant
I. lusitanicum (N = 14) 0.804 0.001 45 <0.001 37 0.060 constant
I. almacai (N = 2) * * * *
I. lemmingii (N = 3) * * * *
A. occidentale (N = 3) * * * *
A. hispanica (N = 5) 0.300 0.624 constant constant constant
Dependent variable: π
L. bocagei (N = 39) 0.074 0.074 133 0.462 154 0.670 94 0.006
A. oligolepis (N = 26) −0.088 0.669 94 0.482 33 0.940 31 0.389
P. duriense (N = 14) 0.251 0.387 30.5 0142 constant constant
P. polylepis (N = 16) 0.257 0.337 constant 18 0.320 1 <0.001
P. willkommii (N = 6) −0.486 0.329 constant constant constant
S. carolitertii (N = 21) 0.226 0.338 63 0.183 12.5 0.700 13.5 0.667
S. pyrenaicus (N = 18) 0.094 0.711 51 0.075 49 0.117 constant
S. torgalensis (N = 1) * * * *
S. aradensis (N = 5) 0.447 0.450 constant constant constant
L. comizo (N = 6) 0.339 0.510 constant 1 0.667 constant
L. sclateri (N = 9) 0.485 0.185 14 0.413 constant constant
I. lusitanicum (N = 14) 0.453 0.120 45 <0.001 30 0.364 constant
I. almacai (N = 2) * * * *
I. lemmingii (N = 3) * * * *
A. occidentale (N = 3) * * * *
A. hispanica (N = 5) 0.1 0.873 constant constant constant
Dependent variable: MNPD
L. bocagei (N = 39) 0.074 0.656 133 0.462 154 0.670 94 0.006
A. oligolepis (N = 26) −0.088 0.669 94 0.484 33 0.940 31 0.389
P. duriense (N = 14) 0.251 0.387 30.5 0.142 constant constant
P. polylepis (N = 16) 0.257 0.337 constant 18 0.320 1 <0.001
P. willkommii (N = 6) −0.486 0.329 constant constant constant
S. carolitertii (N = 21) 0.185 0.422 70 0.128 13.5 0.667 13.5 0.667
S. pyrenaicus (N = 18) −0.125 0.621 41 0.443 51 0.075 constant
S. torgalensis (N = 1) * * * *
S. aradensis (N = 5) 0.200 0.747 constant constant constant
L. comizo (N = 6) 0.395 0.439 constant 1 0.667 constant
L. sclateri (N = 9) 0.485 0.185 14 0.413 constant constant
I. lusitanicum (N = 14) 0.453 0.120 45 <0.001 30 0.364 constant
I. almacai (N = 2) * * * *
I. lemmingii (N = 3) * * * *
A. occidentale (N = 3) * * * *
A. hispanica (N = 5) 0.1 0.873 constant constant constant