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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Biomol NMR. 2015 May 10;62(3):291–301. doi: 10.1007/s10858-015-9944-5

Figure 3.

Figure 3

Aromatic region of the 1H-13C HSQC correlated spectra of MED25 in complex with TAD of VP16. (A) HSQC of uniformly 13C labeled complex. (B) HSQC of alternately 13C labeled complex using 2-13C glycerol as 13C source. (C): Same, but recorded with aromatic TROSY scheme selecting the bottom right TROSY component. The spectra were recorded at 303 K. (D, E and F) Expansions of the region containing the cross peaks of Tyr Hε/Cε, Trp Hζ3/Cζ3 and His Hδ2/Cδ2 recorded with a uniformly 13C-labeled sample (D), an alternately 13C labeled sample using a standard HSQC (middle), and an aromatic TROSY (F). (G, H and I) Expansions of the region containing the cross peaks of Phe Hε/Cε with the corresponding procedures.