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Activity of rationally desgined tRNAUTu variants. For each of sixteen variants, mutations relative to tRNAUTu are depicted in green, with the original variant shown in the upper left. Each variant as well as E. coli SelB and SelA was used to complement FDHHam in E. coli ΔselA ΔselB Δfdhf,. FDHH activity was assessed by appearance of the purple colored reduced BV, with the activity mediated by each variant shown adjacent to it.
