Fig. 2.

Activity of rationally desgined tRNA^UTu variants. For each of sixteen variants, mutations relative to tRNA^UTu are depicted in green, with the original variant shown in the upper left. Each variant as well as E. coli SelB and SelA was used to complement FDH_Ham in E. coli ΔselA ΔselB Δfdhf,. FDH_H activity was assessed by appearance of the purple colored reduced BV, with the activity mediated by each variant shown adjacent to it.