Skip to main content
. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Methods Enzymol. 2016 Jan 13;570:389–420. doi: 10.1016/bs.mie.2015.12.001

Figure 12.

Figure 12

Candidate CRS2 crosslinks between CXCR4 and vMIP-II identified by SDS-PAGE and Western blotting (A) were further subjected to CPM-DSF (B) and SEC (C). Although spatially close and similar in both yield and crosslinking efficiency to the neighboring residues (A), pairs of CXCR4(D187C) with vMIP-II(W5C) and vMIP-II(H6C) stand out as having higher thermal stability (B). Furthermore, the complex with vMIP-II(W5C) has a much better peak shape and height than the complex with vMIP-II(H6C), indicating a higher yield and higher degree of monodispersity of vMIP-II(W5C) than other candidate pairs. Figure adapted from (Qin et al., 2015).