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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Methods Enzymol. 2016 Jan 13;570:389–420. doi: 10.1016/bs.mie.2015.12.001

Figure 5.

Figure 5

Effect of varying the multiplicity of infection (MOI) on the overall levels of expression and the efficiency of crosslinking between a pair of single cysteine mutants of ACKR3 and CXCL12. (A) Non-reducing 10% SDS-PAGE of the samples following metal affinity purification by the tag on the receptor. The lower and the upper bands indicate non-crosslinked receptor and crosslinked complex, respectively. (B) Band densitometry for quantification of receptor expression levels. Receptor yield is expressed as fold increase over the control sample (receptor only, no chemokine). Although the calculated fraction of crosslinked complex is close to 80% across all samples, the receptor yield is maximal at the MOI of 6:4 (receptor:chemokine).