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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Methods Enzymol. 2016 Jan 13;570:389–420. doi: 10.1016/bs.mie.2015.12.001

Figure 8.

Figure 8

SDS-PAGE analysis of a crosslinked complex between ACKR3(Cys) and N-terminal mutants of CXCL12. (A) relative intensity of the bands on a non-reducing and a reducing SDS-PAGE for P2G-CXCL12 (3C), (B) Band intensity from (A) quantified by densitometry. (C) The crosslinking efficiency with ACKR3(Cys) is quantified for cysteine residues introduced at two proximal positions of CXCL12, each in the context of three N-terminally modified chemokine variants; the observed difference in crosslinking efficiency of homologous mutants reflects conformational variations between the chemokine variants.