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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Methods Enzymol. 2016 Jan 13;570:389–420. doi: 10.1016/bs.mie.2015.12.001

Figure 9.

Figure 9

SDS-PAGE and Western blotting analysis of CRS1 disulfide crosslinked complexes of CXCR4 and vMIP-II. Left column: Coomassie stained SDS-PAGE gels of 5 different CXCR4 Cys mutants with 4 different vMIP-II Cys mutants. 2nd column: detection of the same SDS-PAGE gel by Western with an anti-FLAG antibody against a tag on the receptor. 3rd column: detection with an anti-HA antibody against a tag on the chemokine. 4th column: merge of the anti-Flag and anti-HA Westerns. Figure adapted from (Qin et al., 2015).