Female mice from (Figure 1) were split into control SD, exercise SD, control HFD, and exercise HFD groups. (A) Exercise HFD mice weighed significantly less than control HFD mice during mating and gestation. (B) Female mice on HFD had significantly increased daily energy intake compared to those on SD. (C) At mid-gestation, pregnant dams underwent glucose tolerance testing. Control dams on HFD had significantly higher blood glucose levels compared to control mice on the SD after glucose injection. Exercise significantly improved glucose disposal in HFD fed dams when compared to HFD fed control dams. (D) AUC was significantly increased in control HFD dams compared to SD dams, and exercise attenuated this increase. *P < 0.05 and **P < 0.01 compared to control SD; #P < 0.05 and ##P < 0.01 compared to control HFD; n = 11 for control SD, n = 11 for exercise SD, n = 12 for control HFD, and n = 15 for exercise HFD in A – D. Error bars indicate s.e.m.