Fig. 2.

H&E staining, immunohistochemical staining for galectin-3, Iba-1 and GFAP, and apoptotic DNA fragmentation detected by TUNEL staining in a low-power field and a high-power field of the hippocampus at 96 h after ischemic insult. Many damaged CA2 neurons had lost their nuclear affinity for hematoxylin and appeared as an eosinophilic ‘ghost neuron’ in H&E staining. The localization of galectin-3-positive cells coincided with that of Iba-1-positive cells. There were no GFAP-positive cells in the CA2 damaged area. Some of the TUNEL-positive CA2 neurons were distinguishable among the CA1 neurons by the shape of positive stained nuclei. Scale bars in LPF and HPF of H&E staining are 100 and 50 μm, respectively. G3, galectin-3; GF, GFAP; HE, HE staining; HPF, high-power field; Iba, Iba-1; LPF, low-power field; TU, TUNEL staining.