Figure 5.

Resveratrol induces Notch1 expression and lipid accumulation in meibomian gland epithelial cells. Human meibomian gland epithelial cells treated with resveratrol (100 μM) or vehicle control (DMSO) (a) demonstrate increased neutral lipid accumulation in HMGEC, which treatment with resveratrol, Lipitox (green) and DAPI (blue), (b) Increase in fluorescence intensity of Lipitox staining with resveratrol was significant [(**) = (P value = 0.023)]. Error bars represent mean ± SD, (c) Increased mRNA expression of Notch1 and Notch2 as well as increase in FL, NTM, and cleaved Notch1 in resveratrol treated HMGEC compared with control (DMSO). The mRNA expression of Notch3 did not change with resveratrol. (d) Thin layer chromatography (TLC) demonstrated that resveratrol treatment increases sterol esters (neutral lipids) in HMGEC.