Figure 5.

Cytotoxic activity of peptide 2 or control peptide-stimulated PBMC of HLA-B57⁺ melanoma patients and a healthy control donor against WM793 or peptide-pulsed 1205LU target cells. Cytolytic activity of short-term T-cell lines derived from peptide 2 (left panel) or control peptide (right panel) stimulation of PBMC were determined using WM793 (HLA-B57⁺, ■) or peptide 2-pulsed 1205LU target cells (▲) in a standard ⁵¹Cr release assay as in Figure 2. Control-peptide pulsed (∆) or unpulsed (+) 1205 LU cells were used as controls. Results are expressed as mean % cytoxicity ± SE (a–d) and cytotoxic T lymphocyte lysis of WM793 or 1205LU cells pulsed with peptide 2 was significantly different (P < 0.01) from 1205LU cells or control-peptide pulsed target cells. All experiments were repeated at least three times for confirmation.