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. 2015 Feb 18;2:15001. doi: 10.1038/mto.2015.1

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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In vitro characterization of CRAd replication. (a) Relative Luc expression following infection of human cancer cells with either Ad.CMVLuc or Ad.CXCR4Luc. Luciferase activity was measured in cell lysates at 48 hours after infection. Data are presented as mean ± SD. RLUs, relative light units. (b) The CXCR4-to-CMV ratios of Luc expression in human cells following infection with Ad.CMVLuc or Ad.CXCR4Luc. Data points represent the mean ± SD of a representative experiment. (c) Evaluation of CRAd replication. Human colorectal adenocarcinoma LS174T cells were infected with 1 × 10³ vp per cell and harvested on indicated time points. Total DNA was isolated and hexon gene copy number was obtained using quantitative PCR. Data are presented as mean ± SD.