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| Primer | Sequence (5′–3′) |
|---|---|
| NotI-mut-Fa | CTCGGATGGCTAAGGGAGAGCCAGCtagCGCTTCGAGCAGACATG |
| NotI-mut-Ra | CATGTCTGCTCGAAGCGctaGCTGGCTCTCCCTTAGCCATCCGAG |
| Haa-F | ACGCGTAGATCATCGATAATGTCACCACAACGAGACCGGAT |
| Haa-R | CCTAGGATTGCTGTTAGTTTTTTTCATACCTGCAGGCCTA GTTTTCACTA TCAGTG |
| MVF-F | GCATGCTATGAAAAAAACTAACAGATATCACACCGGGAAT CCCAGAATCA |
| MVF-R | TCGATCAGTG GCTCGAGGCATGCCTACCGATATTGTTCGG CCAGAGGGA |
Mutated nucleotides are indicated in lower case.
