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. 2015 Sep 9;2:15014. doi: 10.1038/mto.2015.14

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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OV-loading of CAR-T cells shows minimal changes in T cell viability, CAR expression, or functionality over time. Human HER2-CAR-T cells or CAR-’ve controls were loaded with an MOI of 3 of either VSVΔM51-GFP or vvDD-GFP and monitored for 7 days post-loading for (a) viability, (b) virus replication, (c) CAR expression on CD8+ T cells, (d) CAR expression on CD4+ T cells, or cytokine production (IFNγ and/or TNFα positive cells) by (e) CD4+ or (f) CD8+ T cells via flow cytometry on the days indicated. Data are presented as mean ± SEM from two independent T-cell donors. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. = not significant. CAR, chimeric antigen receptor; MOI, multiplicity of infection; OV, oncolytic virus; VSV, vesicular stomatitis virus; vvDD, “double-deleted” vaccina virus.