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. 2015 Sep 9;2:15014. doi: 10.1038/mto.2015.14

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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OV-loaded CAR-T cells can deposit OV onto tumor targets. (a,b) Murine or (c,d) human CAR-’ve or HER2-CAR T cells were loaded with MOI of 0 or 3 of (a,c) VSVΔM51-GFP or (b,d) vvDD-GFP and co-cultured with D2F2 tumor targets for 24 hours. Virus replication was visualized using the Typhoon Imager to detect GFP signal (left panels). The level of virus replication was quantified using ImageQuant (right panels). Quantification is expressed as mean ± SEM. *P < 0.05, **P < 0.01, n.s. = not significant. Data is representative of 2–3 independent experiments performed in triplicate for each murine and human cells, normalized to tumor-only wells. CAR, chimeric antigen receptor; MOI, multiplicity of infection; n.s., not significant; OV, oncolytic virus; VSV, vesicular stomatitis virus; vvDD, “double-deleted” vaccina virus.