Figure 2.

Sulfoquinovosyl-acylpropanediol (SQAP) switches off tumor angiogenic potential by decreasing HIFα protein levels. (a) Western blots of vascular endothelial cell growth factor, Ang-2, FGF-2, TSP-1, HIF1α, and HIF2α in HAK1-B tumors treated with SQAP (20 mg/kg/day, i.p injection for 21 days). Whole tumors were collected from tumor-bearing mice (n = 4 per group). The band densities for each protein were measured and calibrated by β-actin. All data are represented by mean ± standard deviation. (b) Western blots of vascular endothelial cell growth factor, HIF1α, and HIF 2α for HAK1-B and Huh-7 exposed to SQAP under hypoxic conditions. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were exposed to 3% O₂ hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer.