Figure 4.

ATN-224 rescues copper-mediated inhibition of RAMBO replication in vitro. UM-SCC-74A cells were infected with RAMBO (0.1 MOI) alone or RAMBO (0.1 MOI) incubated with copper ± ATN-224. (a) Representative fluorescent microscopy images of GFP-positive infected cells indicating the presence of viral replication. (b) Quantification of viral titers produced by the indicated SCC cells infected with RAMBO ± copper in the presence or absence of ATN-224 in vitro (0.1 MOI, copper 20 μmol/l + ATN-224 32 μmol/l). Two days postinfection, the virus was harvested from infected cells and quantified by a standard plaque assay on Vero cells. Data shown are representative results from a total of three separate experiments. Data were analyzed by the paired student’s t-test and error bars indicate ± SD for each group. *indicates P value <0.05.